Jump to:
Spinner 000000 16px loading...
Preferred Name

chain termination sequencing assay
Definitions

A DNA sequencing assay which rely on the use of dideoxynucleotides used in 4 distinct sequencing reaction on the same DNA sample. The dideoxynucleotides, once incorporated in the complementary DNA strand being synthesized by the DNA polymerase prevent any further chain elongation. The newly generated sequences are resolved on a polyacrylamide gel using electrophoresis and labels (either fluorochrome or radioactivity) are used to determine the nucleotide present at a given position
ID

http://purl.obolibrary.org/obo/OBI_0000695
alternative term

dye terminator sequencing

Sanger sequencing
definition

A DNA sequencing assay which rely on the use of dideoxynucleotides used in 4 distinct sequencing reaction on the same DNA sample. The dideoxynucleotides, once incorporated in the complementary DNA strand being synthesized by the DNA polymerase prevent any further chain elongation. The newly generated sequences are resolved on a polyacrylamide gel using electrophoresis and labels (either fluorochrome or radioactivity) are used to determine the nucleotide present at a given position
definition source

url:https://en.wikipedia.org/wiki/Sanger_sequencing
editor preferred label

chain termination sequencing assay
example of usage

PMID: 271968. DNA sequencing with chain-terminating inhibitors.
has curation status

http://purl.obolibrary.org/obo/IAO_0000120
has_specified_input

http://purl.obolibrary.org/obo/OBI_0000406
label

chain termination sequencing assay
prefixIRI

OBI:0000695
prefLabel

chain termination sequencing assay
term editor

Philippe Rocca-Serra
subClassOf

http://purl.obolibrary.org/obo/OBI_0000734
Add comment
Add proposal
Delete Subject Author Type Created
No notes to display
Create New Mapping

Delete Mapping To Ontology Source
There are currently no mappings for this class.