loading...| Preferred Name |
enzyme-linked immunospot assay |
| Definitions |
A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest. |
| ID |
http://purl.obolibrary.org/obo/OBI_0600031 |
| alternative term |
ELISPOT assay |
| definition |
A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest. |
| definition source |
IEDB |
| editor preferred label |
enzyme-linked immunospot assay |
| example of usage |
Determination of the frequency of cells producing IFN-gamma in response to viral peptides by placing effector cells on a anti IFN-gamma coated plate, and adding antigen presenting cells pulsed with a peptide. After washing, the bound IFN-gamma is detected with a secondary antibody linked to a dye that visualizes as one spot per cell. |
| has curation status | |
| label |
enzyme-linked immunospot assay |
| prefixIRI |
OBI:0600031 |
| prefLabel |
enzyme-linked immunospot assay |
| term editor |
IEDB |
| subClassOf |
| Delete | Mapping To | Ontology | Source |
|---|---|---|---|
| http://purl.bioontology.org/ontology/MESH/D058501 | Medical Subject Headings / 医学主题词表 | LOOM |