loading...| Preferred Name |
cell line |
| Definitions |
当培养物经过有意的实验过程以建立更均匀和稳定的细胞群时（参见“建立细胞系”），使用术语“系”。这将需要一个或多次传代，但可能涉及额外的选择过程。通过这样的传代和/或选择过程，产生的'细胞系'达到一定水平的遗传稳定性和组成均一性，这在原代培养物中通常是不存在的。由于它们的相对同质性，“细胞系”能够在一段时间内被表征和稳定地增殖。一种新*型*细胞系不仅可以通过原代培养物的传代/选择建立，而且可以通过对现有系列的实验性修饰（例如永生化，稳定的遗传修饰，用于抗性子集的药物选择等）来建立。 这里提供的定义确立了符合细胞系特征的细胞群的“范围” - 特别是那些在培养中具有共同繁殖历史的细胞群。通过这种方式，“细胞系”类划分代表研究人员在科学实践中实际使用的细胞群 - 例如作为培养、实验和分享的投入。定义是这样的，细胞系将显示重要的属性。例如，它们将具有相对均匀的细胞类型组成，因为它们的连续共增殖而经历了类似的选择压力。此外，这些细胞群还可以通过传代数来表征，这也是由于它们共同的传代史。如此处所定义的，“细胞系”可以指活性培养物中，实验性应用的或以静止状态存储以备将来使用的细胞群。 一个培养细胞群体，其代表具有共同增殖历史（即已经在培养中一起连续传代）的遗传稳定且同质的培养细胞群体。 The term 'line' is used when a culture has undergone an intentional experimental process to establish a more uniform and stable population of cells (see 'establishing cell line'). This will require one or more passages, but may involve additional selection processes. Through such passaging and/or selection processes, the resulting 'line' attains some level of genetic stability and compositional homogeneity which is typically absent in primary cultures. Because of their relative homogeneity, ‘lines’ are capable of being characterized and stably propagated over a period of time. A new *type* of cell line can be established not only through the passaging/selection of a primary culture, but also through experimental modifications of existing lines (e.g. immortalization, stable genetic modifications, drug selection for a resistant subset, etc.). The definition provided here establishes the 'scale' of cell populations that qualify as cell lines - specifically those with a shared propagation history in culture. In this way, the 'cell line' class demarcates populations that represent what researchers actually use in the practice of science - e.g. as inputs to culturing, experimentation, and sharing. The definition is such that cell lines will exhibit important attributes. For example, they will have a relatively homogenous cell type composition as they have experienced similar selective pressures due to their continuous co-propagation. In addition, these populations can also be characterized by a passage number, again owing to their common passaging history. As defined here, 'cell line' can refer to a population of cells in active culture, applied experimentally, or stored in a quiescent state for future use. A cultured cell population that represents a genetically stable and homogenous population of cultured cells that shares a common propagation history (i.e. has been successively passaged together in culture). The term 'line' is used when a culture has undergone an intentional experimental process to establish a more uniform and stable population of cells (see 'establishing cell line'). This will require one or more passages, but may involve additional selection processes. Through such passaging and/or selection processes, the resulting 'line' attains some level of genetic stability and compositional homogeneity which is typically absent in primary cultures. Because of their relative homogeneity, ‘lines’ are capable of being characterized and stably propagated over a period of time. A new *type* of cell line can be established not only through the passaging/selection of a primary culture, but also through experimental modifications of existing lines (e.g. immortalization, stable genetic modifications, drug selection for a resistant subset, etc.). The definition provided here establishes the 'scale' of cell populations that qualify as cell lines - specifically those with a shared propagation history in culture. In this way, the 'cell line' class demarcates populations that represent what researchers actually use in the practice of science - e.g. as inputs to culturing, experimentation, and sharing. The definition is such that cell lines will exhibit important attributes. For example, they will have a relatively homogenous cell type composition as they have experienced similar selective pressures due to their continuous co-propagation. In addition, these populations can also be characterized by a passage number, again owing to their common passaging history. As defined here, 'cell line' can refer to a population of cells in active culture, applied experimentally, or stored in a quiescent state for future use. A cultured cell population that represents a genetically stable and homogenous population of cultured cells that shares a common propagation history (i.e. has been successively passaged together in culture). |
| ID |
http://purl.obolibrary.org/obo/CLO_0000031 |
| comment |
当培养物经过有意的实验过程以建立更均匀和稳定的细胞群时（参见“建立细胞系”），使用术语“系”。这将需要一个或多次传代，但可能涉及额外的选择过程。通过这样的传代和/或选择过程，产生的'细胞系'达到一定水平的遗传稳定性和组成均一性，这在原代培养物中通常是不存在的。由于它们的相对同质性，“细胞系”能够在一段时间内被表征和稳定地增殖。一种新*型*细胞系不仅可以通过原代培养物的传代/选择建立，而且可以通过对现有系列的实验性修饰（例如永生化，稳定的遗传修饰，用于抗性子集的药物选择等）来建立。 这里提供的定义确立了符合细胞系特征的细胞群的“范围” - 特别是那些在培养中具有共同繁殖历史的细胞群。通过这种方式，“细胞系”类划分代表研究人员在科学实践中实际使用的细胞群 - 例如作为培养、实验和分享的投入。定义是这样的，细胞系将显示重要的属性。例如，它们将具有相对均匀的细胞类型组成，因为它们的连续共增殖而经历了类似的选择压力。此外，这些细胞群还可以通过传代数来表征，这也是由于它们共同的传代史。如此处所定义的，“细胞系”可以指活性培养物中，实验性应用的或以静止状态存储以备将来使用的细胞群。 The term 'line' is used when a culture has undergone an intentional experimental process to establish a more uniform and stable population of cells (see 'establishing cell line'). This will require one or more passages, but may involve additional selection processes. Through such passaging and/or selection processes, the resulting 'line' attains some level of genetic stability and compositional homogeneity which is typically absent in primary cultures. Because of their relative homogeneity, ‘lines’ are capable of being characterized and stably propagated over a period of time. A new *type* of cell line can be established not only through the passaging/selection of a primary culture, but also through experimental modifications of existing lines (e.g. immortalization, stable genetic modifications, drug selection for a resistant subset, etc.). The definition provided here establishes the 'scale' of cell populations that qualify as cell lines - specifically those with a shared propagation history in culture. In this way, the 'cell line' class demarcates populations that represent what researchers actually use in the practice of science - e.g. as inputs to culturing, experimentation, and sharing. The definition is such that cell lines will exhibit important attributes. For example, they will have a relatively homogenous cell type composition as they have experienced similar selective pressures due to their continuous co-propagation. In addition, these populations can also be characterized by a passage number, again owing to their common passaging history. As defined here, 'cell line' can refer to a population of cells in active culture, applied experimentally, or stored in a quiescent state for future use. The term 'line' is used when a culture has undergone an intentional experimental process to establish a more uniform and stable population of cells (see 'establishing cell line'). This will require one or more passages, but may involve additional selection processes. Through such passaging and/or selection processes, the resulting 'line' attains some level of genetic stability and compositional homogeneity which is typically absent in primary cultures. Because of their relative homogeneity, ‘lines’ are capable of being characterized and stably propagated over a period of time. A new *type* of cell line can be established not only through the passaging/selection of a primary culture, but also through experimental modifications of existing lines (e.g. immortalization, stable genetic modifications, drug selection for a resistant subset, etc.). The definition provided here establishes the 'scale' of cell populations that qualify as cell lines - specifically those with a shared propagation history in culture. In this way, the 'cell line' class demarcates populations that represent what researchers actually use in the practice of science - e.g. as inputs to culturing, experimentation, and sharing. The definition is such that cell lines will exhibit important attributes. For example, they will have a relatively homogenous cell type composition as they have experienced similar selective pressures due to their continuous co-propagation. In addition, these populations can also be characterized by a passage number, again owing to their common passaging history. As defined here, 'cell line' can refer to a population of cells in active culture, applied experimentally, or stored in a quiescent state for future use. |
| editor preferred label |
cell line 细胞系 |
| example of usage |
He, Tong-Chuan, et al., Identification of c-MYC as a target of the APC pathway. Science 281.5382 (1998): 1509-1512.: "为了评估APC的转录效应，我们研究了含有锌诱导型APC基因的人结直肠癌细胞系（HT29-APC）和含有类似诱导型lacZ基因的对照细胞系（HT29-β-Gal）". 请注意，文献中的常见用法通常是“人类结直肠癌细胞系”的形式",如上所见。 但是对"一个细胞系"研究的这种引用，指的是输入培养或实验的离散细胞群体，而不是整个细胞系。 我们将这些离散的群体称为'细胞系'。 He, Tong-Chuan, et al., Identification of c-MYC as a target of the APC pathway. Science 281.5382 (1998): 1509-1512.: "To evaluate the transcriptional effects of APC, we studied a human colorectal cancer cell line (HT29-APC) containing a zinc-inducible APC gene and a control cell line (HT29–β-Gal) containing an analogous inducible lacZ gene". Note that common usage in the literature is often of the form "a human colorectal cancer cell line", as seen above. But such references to studies in "a line" refer to the fact that discrete populations of cells that are input into culturing or experiments, not an entire lineage of cells. It is these discrete populations that we refer to as 'cell lines'. He, Tong-Chuan, et al., Identification of c-MYC as a target of the APC pathway. Science 281.5382 (1998): 1509-1512.: "To evaluate the transcriptional effects of APC, we studied a human colorectal cancer cell line (HT29-APC) containing a zinc-inducible APC gene and a control cell line (HT29–β-Gal) containing an analogous inducible lacZ gene". Note that common usage in the literature is often of the form "a human colorectal cancer cell line", as seen above. But such references to studies in "a line" refer to the fact that discrete populations of cells that are input into culturing or experiments, not an entire lineage of cells. It is these discrete populations that we refer to as 'cell lines'. |
| label |
cell line 细胞系 |
| prefixIRI |
CLO:0000031 |
| prefLabel |
cell line |
| textual definition |
一个培养细胞群体，其代表具有共同增殖历史（即已经在培养中一起连续传代）的遗传稳定且同质的培养细胞群体。 A cultured cell population that represents a genetically stable and homogenous population of cultured cells that shares a common propagation history (i.e. has been successively passaged together in culture). A cultured cell population that represents a genetically stable and homogenous population of cultured cells that shares a common propagation history (i.e. has been successively passaged together in culture). 培养的细胞群体代表具有共同繁殖历史（即已经在培养中一起连续传代）的遗传稳定且同质的培养细胞群体。 A cultured cell population that represents a genetically stable and homogenous population of cultured cells that shares a common propagation history (i.e. has been successively passaged together in culture). |
| 引自 | |
| 术语编辑者 |
MB, SS, JZ, MAH, BP, CS, YH MB, SS, JZ, MAH, BP, CS, YH |
| 编者注 |
In the spring of 2013, a working group comprised of domain experts and representatives from CLO, OBI, CL, and ReO worked to establish a consensus model and definitions of cultured cells across these efforts. This included a careful characterization of how the term 'cell line' should be defined and applied. Notes about this work and its outcomes can be found on the CLO wiki here: http://code.google.com/p/clo-ontology/wiki/Cell_Lines In the spring of 2013, a working group comprised of domain experts and representatives from CLO, OBI, CL, and ReO worked to establish a consensus model and definitions of cultured cells across these efforts. This included a careful characterization of how the term 'cell line' should be defined and applied. Notes about this work and its outcomes can be found on the CLO wiki here: http://code.google.com/p/clo-ontology/wiki/Cell_Lines 在2013年春季，由CLO，OBI，CL和ReO的领域专家和代表组成的工作组经过这些努力中建立了共识模型和培养细胞的定义。 这包括仔细描述术语“细胞系”应该如何定义和应用。 关于这项工作及其结果的说明可以在CLO wiki上找到： http://code.google.com/p/clo-ontology/wiki/Cell_Lines |
| subClassOf |