Preferred Name

nucleic acid uv cross-linking assay

Synonyms

nucl ac uv crosslink

Definitions

Nucleic acids are incubated with purified proteins or a protein mixture and then exposed to a chemical cross-linking agent that may be activated by UV light exposure. The eventual complexes can be identified by sequencing or autoradiography if the nucleic acid is radio-labelled and the sequence is known. The proteins involved in the complex can be recognized by specific antibodies or by retrieving the original protein mixture and carrying further analysis on it.

ID

http://purl.obolibrary.org/obo/MI_0430

definition

Nucleic acids are incubated with purified proteins or a protein mixture and then exposed to a chemical cross-linking agent that may be activated by UV light exposure. The eventual complexes can be identified by sequencing or autoradiography if the nucleic acid is radio-labelled and the sequence is known. The proteins involved in the complex can be recognized by specific antibodies or by retrieving the original protein mixture and carrying further analysis on it.

has_exact_synonym

nucl ac uv crosslink

has_obo_namespace

PSI-MI

id

MI:0430

in_subset

http://purl.obolibrary.org/obo/mi#PSI-MI_slim

label

nucleic acid uv cross-linking assay

notation

MI:0430

prefLabel

nucleic acid uv cross-linking assay

treeView

http://purl.obolibrary.org/obo/MI_0030

subClassOf

http://purl.obolibrary.org/obo/MI_0030

Delete Subject Author Type Created
No notes to display
Create New Mapping

Delete Mapping To Ontology Source
There are currently no mappings for this class.