loading...| Preferred Name |
Polymerase Chain Reaction |
| Synonyms |
Reaction, Polymerase Chain |
| Definitions |
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. |
| ID |
http://purl.bioontology.org/ontology/MESH/D016133 |
| altLabel |
Reaction, Polymerase Chain Anchored PCR Inverse Polymerase Chain Reaction Polymerase Chain Reactions Anchored Polymerase Chain Reaction PCR PCR, Nested Reactions, Polymerase Chain PCR, Inverse Inverse PCR Nested Polymerase Chain Reaction PCR, Anchored Nested PCR |
| AQL |
CL EC ES HI IS MT SN ST TD VE |
| cui |
C0079639 C0032520 C0242574 C0078945 |
| DC |
1 |
| definition |
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. |
| DX |
19910101 |
| FX |
D016324 D022521 |
| HN |
91(90); was see under GENE AMPLIFICATION 1990 |
| Inverse of AQ |
http://purl.bioontology.org/ontology/MESH/Q000639 http://purl.bioontology.org/ontology/MESH/Q000295 http://purl.bioontology.org/ontology/MESH/Q000145 http://purl.bioontology.org/ontology/MESH/Q000941 http://purl.bioontology.org/ontology/MESH/Q000706 http://purl.bioontology.org/ontology/MESH/Q000592 http://purl.bioontology.org/ontology/MESH/Q000191 http://purl.bioontology.org/ontology/MESH/Q000379 |
| Inverse of RO | |
| Machine permutation |
91; was see under GENE AMPLIFICATION 1990 |
| MDA |
19900111 |
| MMR |
20110624 |
| MN |
E05.393.620.500 |
| notation |
D016133 |
| prefLabel |
Polymerase Chain Reaction |
| TERMUI |
T047912 T047909 T047913 T047911 T047908 T047910 T047914 T047915 |
| TH |
NLM (1991) NLM (1990) NLM (1995) |
| tui |
T063 |
| subClassOf |