loading...| Preferred Name |
clip |
| Synonyms |
cross-linking immunoprecipitation clip CLIP cross linking immunoprecipitation |
| Definitions |
Combination of cross-linking and co-immunoprecipitation aimed to find protein-RNA interactions. The canonical method uses first a cross-linking procedure over a tissue sample or lysate, and the immunoprecipitated with specific antibodies for the protein of interest. Unspecific proteins are digested via proteinase K treatment. RNA can be then identified via Northern blotting or using RT-PCR and then sequencing of the generated cDNA. https://en.wikipedia.org/wiki/CLIP |
| ID |
http://purl.obolibrary.org/obo/MI_2191 |
| created_by |
ppm |
| creation_date |
2015-06-15T10:17:34Z |
| definition |
Combination of cross-linking and co-immunoprecipitation aimed to find protein-RNA interactions. The canonical method uses first a cross-linking procedure over a tissue sample or lysate, and the immunoprecipitated with specific antibodies for the protein of interest. Unspecific proteins are digested via proteinase K treatment. RNA can be then identified via Northern blotting or using RT-PCR and then sequencing of the generated cDNA. https://en.wikipedia.org/wiki/CLIP |
| has_exact_synonym |
cross-linking immunoprecipitation clip CLIP cross linking immunoprecipitation |
| has_obo_namespace |
PSI-MI |
| id |
MI:2191 |
| in_subset | |
| label |
clip |
| notation |
MI:2191 |
| prefLabel |
clip |
| treeView | |
| subClassOf |